Stagetop Incubator BL3

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Bold Line 3
Stagetop Incubator

Exclusive Full Sample Feedback Technology, the benchmark for high-end labs and core facilities.

BL3 is the first microscope incubation solution to provide closed-loop feedback control at the sample level for temperature, humidity, and gas composition, ensuring higher experimental reliability, lower gas consumption and error-proof workflows.

  • Complete Sample Feedback.
  • Advanced digital gas mixing and humidity comes standard.
  • Excellent thermal distribution across multi-well plate.
  • Versatile and flexible configurations for your microscope and experimental need.
COMPONENTS:

Chamber

The stagetop chamber guarantees superior environmental control around your sample. Modular inserts adapt to multiple stage geometries.

  • Models available for all XY stages/piezos on the market, including upright microscopes.
  • Wireless heated sliding lid – low profile for Koehler compatibility.
  • Can natively accommodate multi-well plates without additional inserts.
  • Universal Sample Holders  can be combined with a simple adapter for use in the XY stage/piezo without incubation.
  • In-chamber sensor for complete sample feedback provides an unprecedented level of accuracy and repeatability – ensures reliable environment where it matters most.
  • Perfusion holes for inlet and outlet of microfluidic tubes.

Universal Inserts

  • Universal wellplate size fits most microscope stages for fixed or live cell imaging.

  • Quickly exchange inserts for dishes, slides and plates.

  • Optimized airflow and thermal stability.

Controllers

The BL3 Controllers are designed to deliver precise and reliable environmental control inside the cage incubator, ensuring optimal conditions for long-term live-cell imaging.

Each configuration combines advanced temperature regulation with optional closed-loop CO₂ and O₂ gas control, allowing users to choose the level of environmental management that best suits their application.

Available Product Bundle

  • Temperature range: from 3°C above ambient to 45°C
  • Temperature accuracy: ± 0.1°C
  • Low energy consumption thanks to warm air recirculation
  • One-touch control of all environmental parameters from a single intuitive touchscreen panel, powered by intelligent software that automatically regulates the system

Delivers combined control of temperature and CO₂ concentration for standard live-cell imaging applications.

  • Above temperature specifications
  • CO₂ range: 0 – 20 %
  • CO₂ accuracy: ± 0.1 %
  • CO₂ sensor: NDIR dual-wavelength, 10-year lifetime
  • Air pump flow rate: 0.05 – 0.4 l/min (up to 1l/min with compressed air tank)

Adds oxygen control for hypoxia, anoxia, and normoxia studies, expanding the system’s experimental versatility

  • CO₂ range: 0 – 20 %, accuracy ± 0.1 %
  • O₂ range: 0 – 21 %, accuracy ± 0.1 % at 5 % O₂
  • O₂ sensor: Optical, 5-year lifetime
  • Air pump flow rate: 0.05 – 0.4 l/min (up to 1l/min with compressed air tank)

Provides full oxygen control for advanced anoxia, hypoxia, and hyperoxia applications.

  • CO₂ range: 0 – 20 %, accuracy ± 0.1 %
  • O₂ range: 0 – 95 %, accuracy ± 0.1 %
  • Gas mixing via digital mass flow controllers for N₂, O₂, CO₂
  • Air pump flow rate (when using Air/CO2 Mode) : 0.2 – 0.4 l/min (factory set 0.4 l/min)

Active Humidity Controller

Independent humidity module keeps chamber relative humidity stable and protects samples during long imaging sessions. Digital humidity sensor provides active feedback.

  • Controls relative humidity inside the stagetop chamber in the range 50% to 95% at 37°C. 
  • Choose the preferred humidity level in 1% increments easily, using OKO-TOUCH interface.
  • External humidity module – NO water moat. Keeps water safely away from your microscope.
  • Water heater humidifies the gas mixture.
  • Tube heater prevents condensation and maintains humidity level between water heater and chamber.

Touch Screen Interface

One-touch control of all environmental parameters from a single intuitive touchscreen panel, powered by intelligent software that automatically regulates the system.

Full Sample Feedback Technology

The CO2-H-IN-CHAMBER SENSOR In-Chamber for closed-loop CO2 and Humidity feedback at the sample level, ensuring improved accuracy for reliable and ERROR-PROOF experiments. SET IT, RUN IT, TRUST IT — no surprises the next morning.

  • CO₂ measurement range: 0–20%, accuracy ±0.1%
  • Humidity measurement range: 50–99%, accuracy ±3%
  • If used along with the CO2/Hypoxia controller, it also provides a calculation of the O2 level. 
  • Sensor’s lifetime: 5+ years

Available Product Codes

Available Packages
Okolab Codes
Temperature controller only
Temperature and CO2 gas controller
Temperature and CO2-O2 hypoxia
Temperature and CO2-O2 hyperoxia

Every microscope setup is unique, so is your Okolab solution.​

Request a personalized quote and get expert guidance on the ideal BL3 configuration for your system.