Heating and Cooling
Stagetop Incubator
Water Jacket Incubating Chamber with External Water Bath
Water Jacket Incubator
Okolab’s Water Jacket Incubating Chamber provides rock-solid incubation stability both above and below room temperature, thanks to the thermal properties of water.
Water is pumped from an external bath into the chamber’s base and lid. The actual sample temperature is monitored inside the chamber, using the provided fine gauge thermocouple. The temperature controller uses this thermal feedback from the sample to update the water bath’s set point, ensuring a specimen temperature accuracy of ± 0.1°C.
Features
- Temperature Range: from 10–15 °C below ambient (model dependent) up to 60 °C
- Stable Sample Temperature: ± 0.1 °C accuracy using Sample Feedback Mode
- Chamber Feedback Mode Option: ± 0.3 °C when room temperature remains stable; no temperature probe required
- Water Circulation: occurs in both base and lid to minimize gradients
- Modular Inserts: magnetic inserts support petri dishes, chamber slides, and multi-well plates
- Perfusion Ports: built-in tubing inlets/outlets for microfluidics and perfusion applications
Temperature Controllers
Choose from two temperature controller options, depending on the required thermal range:
H101-BASIC-BL
T range [3°C above room; 60°C]
Features
Temperature Range: from 3 °C above ambient to 60 °C
Feedback Modes: sample or chamber
Accuracy: ± 0.1 °C (sample); ± 0.3 °C (chamber)
Touch Control: intuitive OKO‑TOUCH interface
Compatible With:
- Digital gas mixers(± 0.1 %)
- Manual gas mixers (± 1 %)
- Open-loop humidity module (±80 % efficiency)
- Active humidity controller (≥95% RH)
- Water jacket objective collar
- SMART BOX Data Logger/Remote Control systems
H101-CRYO-BL
T range [5°C; 60°C]
Features
Temperature Range: from below ambient (approx. 5 °C depending on chamber) to 60 °C
Feedback Modes: sample or chamber
Compatible With:
- Digital gas mixers(± 0.1 %)
- Manual gas mixers (± 1 %)
- Open-loop humidity module (±80 % efficiency)
- Active humidity controller (≥95% RH – only for use above ambient)
- Water jacket objective collar
- SMART BOX Data Logger/Remote Control systems
Gas Control: CO2 and O2 Precision
Digital Bold Line Gas Mixers connect directly to temperature controllers and are managed through the same user interface (OKO‑TOUCH).
Features
- CO2 and O2 Accuracy: ± 0.1 %
- Adjustable Output Flow: programmable via touch interface
- Programmable Gas Cycles: for complex protocols
- Optional Active Humidity Control: compatible with Bold Line systems
- Data Logging & Software Integration: USB, SMART BOX, and third‑party microscopy platforms
- Optional Compressed Air using Okolab air pump
- PTFE Filter: removes particulates from gas streams
Gas Option
Okolab Codes
Digital CO2
Digital CO2-O2 (hypoxia and hyperoxia)
Manual CO2
2GF-MIXER
Manual CO2-O2 (hypoxia)
3GF MIXER-HYPOXIA
Manual CO2-O2 (hyperoxia)
3GF MIXER-HYPEROXIA
Humidity Module
Gas is humidified through a bubbling column immersed in the water bath, with a pre‑heating coil to prevent condensation and ensure thermal equilibrium before entering the incubation chamber.
- Can be used in cooling and heating modes
- Open-loop, 80 % relative humidity to the sample
OKO‑TOUCH User Interface
The OKO‑TOUCH touchscreen provides centralized control of all connected Bold Line controllers.
It automatically detects system components and displays supported functions, including:
Features
System power control
Set point definition (temperature and gas)
Flow rate adjustment
Temperature and gas cycle programming
Feedback mode selection (sample vs chamber)
Calibration routines
Display of all measured paramenters
- Onboard Memory (Internal Data Logger)