H203-SUPER-Z

BL3 – Gas chamber for Leica Super Z Galvo Stage
The chamber fits on the XY stage and covers the Super Z galvo stage (SP5/SP8). It host MW plates without addition of any insert.
A high precision temperature sensor is embedded the chamber to provide the best temperature conterol accuracy on the sample.

Perfusion holes:# 4, 2.5 mm I.D.
Minimum condenser working distance: 26 mm.
Total weight on galvo stage: 80 g.
Compatible with the CO2-H-IN-CHAMBER SENSOR for in chamber closed loop control on CO2 and Humidity.
It requires at least one specimen holder.

An example of why in-chamber feedback is important.

Traditional incubation systems with digital CO2 mixers sample CO2 concentration at the source, the mixer itself, where it is later routed to the live cell chamber.  In theory, this should produce an environment within the chamber that matches the defined CO2 setpoint of the mixer; in reality, this is not always the case.

The reality is that in-chamber values of CO2 can differ significantly from the setpoint of an externally monitored digital mixer; usually due to some restriction of airflow or excessive leakage of CO2-mixed air from the chamber.  In some cases, the loss of air to (or from) the chamber is severe enough to cause experimental failure.

Some common causes for this, observed multiple times in the field, are the following:

  • an improperly secured lid on the microenvironment chamber
  • loose connections or kinks in air tubing downstream from the mixer
  • improper placement of sample or gaps between the sample carrier (dish, slide, etc) and the microenvironment chamber sample insert
  • leaving a position on a multi-sample insert open to ambient air

By bringing feedback inside the chamber, the BL3 ‘sees’ what no other live cell chamber today can, ensuring accurate and stable conditions right from the start of your experiment, right where your experiment is happening.